IL-17F Monoclonal / Alexa Fluor 647 / 1058A

Product Details
Supplier R&D Systems
Catalog #: IC20571R-025 (View supplier product page)
Size 25 Tests
Price $179.00
Antigen IL-17F
Clone 1058A
Host Rabbit
Isotype IgG
Conjugate Alexa Fluor 647
Target Species Mouse
Applications ICS
Description Mouse IL-17F Alexa Fluor 647-conjugated Antibody
About IL-17F and Alexa Fluor 647
IL-17F The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
Images
  IL-17F Alexa Fluor 647 R&D Systems antibody anti target:Mouse host:Rabbit isotype:IgG clone:1058A Detection of IL-17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse...

Detection of IL-17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC20571R) or (B) Normal Rabbit IgG Alexa Fluor® 647-conjugated Control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Alexa Fluor 647 Excitation and Emission Spectra