GPR34 Monoclonal / Unconjugated / 419859
GPR34 Monoclonal / Unconjugated / 419859
Product Details
Supplier | R&D Systems | |
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Catalog #: | MAB4617-SP (View supplier product page) | |
Size | 25 μg | |
Price | $149.00 | |
Antigen | GPR34 | |
Clone | 419859 | |
Host | Mouse | |
Isotype | IgG2a | |
Conjugate | Unconjugated | |
Target Species | Human | |
Applications | CyTOF, IHC, FC, ICFC, ICC | |
Description | Human GPR34 Antibody Intracellular Flow Cytometry = ICFC |
About GPR34 and Purified
GPR34 | G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.[supplied by OMIM, Apr 2006] |
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Images
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Detection of GPR34 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
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GPR34 in A172 Human Cell Line. GPR34 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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