FluoroFinder

	Supplier	R&D Systems
	Catalog #:	MAB560 (View supplier product page)
	Size	500 μg
	Price	$549.00
	Antigen	GFR alpha-1
	Clone	81401
	Host	Mouse
	Isotype	IgG2b
	Conjugate	Unconjugated
	Target Species	Mouse, Rabbit, Rat
	Applications	WB, IHC, FC, IHC-Fr, Block/Neutralize, ICC
	Description	Rat GFR alpha-1/GDNF R alpha-1 Antibody

	GFR alpha-1	This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

		Detection of Rat GFR alpha-1/GDNF R alpha-1 by Western Blot. Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFR alpha-1/GDNF R alpha-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFR alpha-1/GDNF R alpha-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1.
		GFR alpha-1/GDNF R alpha-1 in Rat Spinal Cord. GFR alpha-1/GDNF R alpha-1 was detected in perfusion fixed frozen sections of rat spinal cord using Rat GFR alpha-1/GDNF R alpha-1 Monoclonal Antibody (Catalog # MAB560) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.