FluoroFinder

	Supplier	R&D Systems
	Catalog #:	AF5647 (View supplier product page)
	Size	100 μg
	Price	$479.00
	Antigen	FOXP2
	Clone
	Host	Sheep
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human, Mouse, Rat
	Applications	WB, IHC, ICC
	Description	Human/Mouse FoxP2 Antibody

	FOXP2	This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and adult brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Multiple alternative transcripts encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

		Detection of Human FoxP2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
		FoxP2 in HeLa Human Cell Line. FoxP2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.