Fibroblast Activation Protein alpha Polyclonal / Unconjugated /
Fibroblast Activation Protein alpha Polyclonal / Unconjugated /
Product Details
| Supplier | R&D Systems | |
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| Catalog #: | AF3715-SP (View supplier product page) | |
| Size | 25 μg | |
| Price | $169.00 | |
| Antigen | Fibroblast Activation Protein alpha | |
| Clone | ||
| Host | Sheep | |
| Isotype | IgG | |
| Conjugate | Unconjugated | |
| Target Species | Human, Mouse | |
| Applications | WB, IHC, IP, Knockout Validated, FC, IHC-P, ICC, In-situ Hybridization | |
| Description | Human Fibroblast Activation Protein alpha /FAP Antibody |
About Fibroblast Activation Protein alpha and Purified
| Fibroblast Activation Protein alpha | The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
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Images
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Fibroblast Activation Protein alpha/FAP in Human Basal Cell Carcinoma. Fibroblast Activation Protein alpha/FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Fibroblast Activation Protein alpha/FAP in Human Squamous Cell Carcinoma. Fibroblast Activation Protein alpha/FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Detection of Human Fibroblast Activation Protein alpha/FAP by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein alpha/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
Citations
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