ESGP in Mouse Testis. ESGP was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to late spermatids. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse ESGP by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ESGP at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ESGP in D3 Mouse Cell Line. ESGP was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.