Detection of Human, Mouse, and Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.