FluoroFinder

	Supplier	R&D Systems
	Catalog #:	MAB666-500 (View supplier product page)
	Size	500 μg
	Price	$1,569.00
	Antigen	CD226
	Clone	102511
	Host	Mouse
	Isotype	IgG1
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, FC, CyTOF, Block/Neutralize
	Description	Human DNAM-1/CD226 Antibody

	CD226	This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

		Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666). The ND₅₀ is typically 0.1-0.6 µg/mL.
		Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3^- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).