IL-8 Polyclonal / Unconjugated /
IL-8 Polyclonal / Unconjugated /
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | AF1608 (View supplier product page) | |
| Size | 100 μg | |
| Price | $459.00 | |
| Antigen | IL-8 | |
| Clone | ||
| Host | Goat | |
| Isotype | IgG | |
| Conjugate | Unconjugated | |
| Target Species | Canine | |
| Applications | WB, Block/Neutralize, ICC | |
| Description | Canine IL-8/CXCL8 Antibody |
About IL-8 and Purified
| IL-8 | The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020] |
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Images
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Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Canine IL-8/CXCL8 Antibody. Recombinant Canine IL-8/CXCL8 (Catalog # 1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1608). The ND50 is typically 0.1-0.5 µg/mL. |
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IL-8/CXCL8 in Canine PBMCs. IL-8/CXCL8 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A using Goat Anti-Canine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1608) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
Citations
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Purified Excitation and Emission Spectra
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