FluoroFinder

	Supplier	R&D Systems
	Catalog #:	AF376-SP (View supplier product page)
	Size	25 μg
	Price	$149.00
	Antigen	CCL27
	Clone
	Host	Goat
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human
	Applications	WB, IHC, Block/Neutralize, IHC-P, IHC-Fr
	Description	Human CCL27/CTACK Antibody

	CCL27	This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Sep 2014]

		CCL27/CTACK in Human Skin. CCL27/CTACK was detected in immersion fixed frozen sections of human skin using Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF376) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
		Chemotaxis Induced by CCL27/CTACK and Neutralization by Human CCL27/CTACK Antibody. Recombinant Human CCL27/CTACK (Catalog # 376-CT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR10/GPR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF376). At 55 µg/mL, this antibody will neutralize > 70% of the chemotactic effect.