Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Calcineurin B Monoclonal Antibody (Catalog # MAB1348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.