FluoroFinder

	Supplier	R&D Systems
	Catalog #:	AF8299-SP (View supplier product page)
	Size	25 μg
	Price	$149.00
	Antigen	CD239
	Clone
	Host	Goat
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Mouse, Rat
	Applications	WB, IHC, IF
	Description	Mouse/Rat BCAM Antibody

	CD239	This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

		BCAM in Mouse Liver. BCAM was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
		Detection of Mouse and Rat BCAM by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, and rat lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BCAM at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.