B7-H7/HHLA2 in Human Monocytes. B7-H7/HHLA2 was detected in immersion fixed human monocytes using Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human B7-H7/HHLA2 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidny cell line transfected with human B7-H7. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.