FluoroFinder

	Supplier	R&D Systems
	Catalog #:	MAB7144-SP (View supplier product page)
	Size	25 μg
	Price	$159.00
	Antigen	B7-H6
	Clone	875001
	Host	Mouse
	Isotype	IgG1
	Conjugate	Unconjugated
	Target Species	Human
	Applications	FC, ELISA, CyTOF, Knockout Validated
	Description	Human B7-H6 Antibody

	B7-H6	B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]

		Detection of B7-H6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
		Human B7-H6 ELISA Standard Curve. Recombinant Human B7-H6 protein was serially diluted 2-fold and captured by Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB71442) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
		B7-H6 Specificity is Shown by Flow Cytometry in Knockout Cell Line. B7-H6 knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the B7-H6 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.