Perforin Monoclonal / Alexa Fluor 647 / CB5.4

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP1-97512AF647 (View supplier product page)
Size 0.1 ml
Price $609.00
Antigen Perforin
Clone CB5.4
Host Rat
Isotype IgG2a
Conjugate Alexa Fluor 647
Target Species Mouse
Applications WB, FC, ICC/IF, IHC-P, IHC-Fr, IHC
Description Recognizes mouse perforin (region aa 402-534).
About Perforin and Alexa Fluor 647
Perforin This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
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Alexa Fluor 647 Excitation and Emission Spectra