CD15 / PE / VIMC6

Citations: (5)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-113-485 (View supplier product page)
Size 100 tests in 200 µL
Price $325.00
Antigen CD15
Clone VIMC6
Host Mouse
Isotype IgM
Conjugate PE
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), FC, IF, IHC
Description The CD15 antibody recognizes the CD15 antigen which is expressed on human myelomonocytic cells. It is present on neutrophils, eosinophils, and some monocytes, masked on basophils, but not on lymphocytes. The antigen is also expressed on Hodgkin/Reed-Sternberg cells. | The CD15 antibody recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. This structure is also known as stage specific embryonic antigen 1 (SSEA-1) and a common surface marker for pluripotency on mouse ES and iPS cells. On human cells in contrast, SSEA-1 is expressed only on early differentiating cells. The CD15 antibody recognizes the SSEA-1 antigen in both species. For applications in ES and iPS research, the dilution recommended in the data sheet may need adjustment.
About CD15 and PE
CD15 The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Citations
PMID 21372811
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PE Excitation and Emission Spectra