CD5 / Brilliant Violet 421 / L17F12

Product Details
Supplier BioLegend
Catalog #: 364029 (View supplier product page)
Size 25T
Price $187.00
Antigen CD5
Clone L17F12
Host Mouse
Isotype IgG2a κ
Conjugate Brilliant Violet 421
Target Species Human
Applications FC
Description Brilliant Violet 421 anti-human CD5
About CD5 and Brilliant Violet 421
CD5 This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
Brilliant Violet 421 Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD5 Brilliant Violet 421 BioLegend antibody anti target:Human host:Mouse isotype:IgG2a κ clone:L17F12

Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ anti-human CD5 (clone L17F12, filled histogram) or Brilliant Violet 421™ mouse IgG2a, κ isotype control (open histogram).

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Brilliant Violet 421 Excitation and Emission Spectra