IgM Secondary Antibody / Alexa Fluor 647 / MRM-47

Citations: (1)
Product Details
Supplier BioLegend
Catalog #: 408909 (View supplier product page)
Size 25 μg
Price $101.00
Antigen IgM
Clone MRM-47
Host Mouse
Isotype IgG
Conjugate Alexa Fluor 647
Target Species Rat
Applications FC, IHC-Fr
Description Alexa Fluor 647 anti-IgM
About IgM and Alexa Fluor 647
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
Images
  IgM Alexa Fluor 647 BioLegend antibody anti target:Rat host:Mouse isotype:IgG clone:MRM-47

Lewis rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 647(filled histogram), or rat IgM isotype control(open histogram).
  IgM Alexa Fluor 647 BioLegend antibody anti target:Rat host:Mouse isotype:IgG clone:MRM-47

C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 647 (red) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(blue) at room temperature for 2 hours. The image was captured by 10X objective.
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Alexa Fluor 647 Excitation and Emission Spectra