Fucosyltransferase 3/5 / Alexa Fluor 594 / NCRC37
Fucosyltransferase 3/5 / Alexa Fluor 594 / NCRC37
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 392603 (View supplier product page) | |
| Size | 25 μg | |
| Price | $159.00 | |
| Antigen | Fucosyltransferase 3/5 | |
| Clone | NCRC37 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Alexa Fluor 594 | |
| Target Species | Human | |
| Applications | IHC-P | |
| Description | Alexa Fluor 594 anti-human CD174 |
About Fucosyltransferase 3/5 and Alexa Fluor 594
| Fucosyltransferase 3/5 | Enables 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity and 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity. Involved in ceramide metabolic process and oligosaccharide metabolic process. Predicted to be located in Golgi membrane. [provided by Alliance of Genome Resources, Apr 2022] | |
|---|---|---|
| Alexa Fluor 594 | Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive. |
Images
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Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0 M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes permeabilized with 0.5% TritonX-100 in PBS for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 goat anti-Vimentin (Clone O91D3) antibody (green) and Alexa Fluor® 594 anti-human CD174 (Clone NCRC37) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software. |
Citations
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Reviews & Ratings
Alexa Fluor 594 Excitation and Emission Spectra
$159.00
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