CD15 Monoclonal / PE / REA321

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-117-801 (View supplier product page)
Size 30 tests in 60 µL
Price $130.00
Antigen CD15
Clone REA321
Host Human
Isotype IgG1
Conjugate PE
Target Species Human, Mouse
Applications MICS (MACSima Imaging Cyclic Staining), FC, IF, IHC, Mass Cytometry
Description Clone REA321 recognizes the human and mouse stage specific embryonic antigen 1 (SSEA-1) antigen, which is a Lewis blood group related carbohydrate antigen, also known as CD15. SSEA-1 is expressed on human myelomonocytic cells and is present on neutrophils, eosinophils, and some monocytes, but not on basophils or lymphocytes. The antigen is also expressed on Hodgkin/Reed-Sternberg cells. SSEA-1 is a common surface marker for pluripotency on mouse ES and iPS cells. On human cells in contrast, SSEA-1 is expressed only on early differentiating cells. | Additional information: Clone REA321 displays negligible binding to Fc receptors.
About CD15 and PE
CD15 The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Citations
Reviews & Ratings
PE Excitation and Emission Spectra