IgM Secondary Antibody / Vio Bright 515 / REA979

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-116-317 (View supplier product page)
Size 100 tests in 200 µL
Price $235.00
Antigen IgM
Clone REA979
Host Human
Isotype IgG1
Conjugate Vio Bright 515
Target Species Mouse
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Description Clone REA979 recognizes the mouse IgM antigen. The antibody is suited for the indirect immunofluorescent staining and flow cytometric analysis of cells stained with primary mouse antibodies of IgM isotype and can further be used for flow cytometric analysis of mouse B cells expressing surface IgM. | Additional information: Clone REA979 displays negligible binding to Fc receptor
About IgM and Vio Bright 515
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Vio Bright 515 Vio®Bright® B515 from Miltenyi has an excitation peak at 488 nm and an emission peak at 514. It is comparable to BB515 in flow cytometry.
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Vio Bright 515 Excitation and Emission Spectra