CD86 Monoclonal / CF405S / C86/1146

Product Details
Supplier Biotium
Catalog #: BNC041146-500 (View supplier product page)
Size 500 μl
Price $551.00
Antigen CD86
Clone C86/1146
Host Mouse
Isotype IgG1 κ
Conjugate CF405S
Target Species Human, Mouse, Rat
Applications IF, IHC, IHC-P, WB
Description Recognizes a protein of 70 kDa, which is identified as CD86. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4, a cancer immunotherapy target. It is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin€s disease. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
About CD86 and CF405S
CD86 This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
CF405S CF®405S from Biotium is a fluorophore with an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to Alexa Fluor™ 405. It can be use in fluorescence microscopy, specifically its been validated in Structured Illumination (SIM), and flow cytometry. However, in flow cytometry, superior alternatives are Brilliant™ Violet 421, SuperNova v428 and StarBright Violet 440.
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CF405S Excitation and Emission Spectra