CD324 Monoclonal / CF488A / CDH1/1525

Product Details
Supplier Biotium
Catalog #: BNC881525-500 (View supplier product page)
Size 500 μl
Price $551.00
Antigen CD324
Clone CDH1/1525
Host Mouse
Isotype IgG1 κ
Conjugate CF488A
Target Species Human
Applications IF, IHC, IHC-P, WB
Description This antibody recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
About CD324 and CF488A
CD324 This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
CF488A CF®488A from Biotium is a fluorophore with an excitation peak at 490 nm and an emission peak at 515 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry. In microscopy, CF®488A has been specifically validated for STORM, STED, Structured Illumination (SIM), 2-Photon microscopy and TIRF.
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CF488A Excitation and Emission Spectra