Involucrin Monoclonal / CF568 / SY5

Product Details
Supplier Biotium
Catalog #: BNC680678-500 (View supplier product page)
Size 500 μl
Price $551.00
Antigen Involucrin
Clone SY5
Host Mouse
Isotype IgG1 κ
Conjugate CF568
Target Species Canine, Gorilla, Human, Monkey, Owl, Porcine
Applications IHC, IHC-P
Description This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
About Involucrin and CF568
Involucrin Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
CF568 CF®568 from Biotium is a fluorophore with an excitation peak at 562 nm and an emission peak at 583 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 568 and ATTO 565. However, in flow cytometry, a superior alternative is R-PE. In microscopy, CF®568 is validated in super-resolution imaging by STORM, STED, Structured Illumination (SIM) and TIRF.
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CF568 Excitation and Emission Spectra