FluoroFinder

	Supplier	BioLegend
	Catalog #:	367424 (View supplier product page)
	Size	100T
	Price	$402.00
	Antigen	PD-1
	Clone	NAT105
	Host	Mouse
	Isotype	IgG1 κ
	Conjugate	Brilliant Violet 510
	Target Species	Human
	Applications	FC
	Description	Brilliant Violet 510 anti-human CD279 (PD-1)

	PD-1	Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020]
	Brilliant Violet 510	Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due to its improved signal-to-noise over alternatives like V500 and AmCyan. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.

		Human peripheral blood lymphocytes were stained with CD3 PE and CD279 (clone NAT105) Brilliant Violet 510™ (top), or mouse IgG1, κ Brilliant Violet 510™ isotype control (bottom).

		PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD279 (clone NAT105) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).