NR1D2 / Alexa Fluor 594 / W15136A

Product Details
Supplier BioLegend
Catalog #: 689403 (View supplier product page)
Size 25 μg
Price $159.00
Antigen NR1D2
Clone W15136A
Host Rat
Isotype IgG1 κ
Conjugate Alexa Fluor 594
Target Species Human, Mouse
Applications ICC
Description Alexa Fluor 594 anti-NR1D2
About NR1D2 and Alexa Fluor 594
NR1D2 This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
Alexa Fluor 594 Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive.
Images
  NR1D2 Alexa Fluor 594 BioLegend antibody anti target:Human, Mouse host:Rat isotype:IgG1 κ clone:W15136A

HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with either (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 594 Rat IgG1, or Alexa Fluor® 594 anti-NR1D2 antibody (red) at (B) 1: 100 (5 µgg/ml), (C) 1: 250 (2 µg/ml) and (D) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). Actin filaments were labeled with Flash Phalloidin™ Green 488 (green). The image was captured with a 60X objective.
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Alexa Fluor 594 Excitation and Emission Spectra