Galectin-9 / Alexa Fluor 594 / 9M1-3
Galectin-9 / Alexa Fluor 594 / 9M1-3
Citations:
(1)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 348921 (View supplier product page) | |
| Size | 25 μg | |
| Price | $159.00 | |
| Antigen | Galectin-9 | |
| Clone | 9M1-3 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Alexa Fluor 594 | |
| Target Species | Human | |
| Applications | IHC-P | |
| Description | Alexa Fluor 594 anti-human Galectin-9 |
About Galectin-9 and Alexa Fluor 594
| Galectin-9 | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] | |
|---|---|---|
| Alexa Fluor 594 | Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive. |
Images
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Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris Buffered 1X (1.0 M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human Galectin-9 (clone 9M1-3, red) and Alexa Fluor® 647 anti-Vimentin (clone O91D3, green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software. |
Citations
| PMID 23180819 | ||
|---|---|---|
| Additional Sources |
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Reviews & Ratings
Alexa Fluor 594 Excitation and Emission Spectra
$159.00
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