CD71 / FITC / 8D3
CD71 / FITC / 8D3
Citations:
(10)
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA2474F (View supplier product page) | |
| Size | 100 μg | |
| Price | $316.00 | |
| Antigen | CD71 | |
| Clone | 8D3 | |
| Host | Rat | |
| Isotype | IgG2a | |
| Conjugate | FITC | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | RAT ANTI MOUSE CD71:FITC |
About CD71 and FITC
| CD71 | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Images
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Figure A. RPE conjugated rat anti mouse TER-119 (MCA6044PE) and FITC conjugated Rat IgG2a isotype control (MCA1212F) Figure B. RPE conjugated rat anti mouse TER-119 (MCA6044PE) and FITC conjugated rat anti mouse CD71 (MCA2474F). All experiments performed on mouse bone marrow. Data acquired on the ZE5™ Cell Analyzer |
Citations
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Reviews & Ratings
FITC Excitation and Emission Spectra
$316.00
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