CD31 / FITC / ER-MP12
CD31 / FITC / ER-MP12
Citations:
(36)
Product Details
| Supplier | Bio-Rad | |
|---|---|---|
| Catalog #: | MCA2388F (View supplier product page) | |
| Size | 100 μg | |
| Price | $316.00 | |
| Antigen | CD31 | |
| Clone | ER-MP12 | |
| Host | Rat | |
| Isotype | IgG2a | |
| Conjugate | FITC | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | RAT ANTI MOUSE CD31:FITC |
About CD31 and FITC
| CD31 | The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Images
|
Figure A. RPE conjugated rat anti mouse CD45R (MCA1258PE) and FITC conjugated rat IgG2a isotype control (MCA6005F) Figure B. RPE conjugated rat anti mouse CD45R (MCA1258PE) and FITC conjugated rat anti mouse CD31 (MCA2388F). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5™ cell analyzer. |
Citations
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FITC Excitation and Emission Spectra
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