CD117 / Brilliant Ultraviolet 395 / YB5.B8

Citations: (1)
Product Details
Supplier BD Biosciences
Catalog #: 745740 (View supplier product page)
Size 50 μg
Price $538.00
Antigen CD117
Clone YB5.B8
Host Mouse
Isotype IgG1 κ
Conjugate Brilliant Ultraviolet 395
Target Species Human
Applications FC
Description Hu CD117 BUV395 YB5.B8 50ug
About CD117 and Brilliant Ultraviolet 395
CD117 This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]
Brilliant Ultraviolet 395 Brilliant™ UltraViolet 395 (BUV395) is a UV-emitting base polymer dye that can be excited by the 355 nm UV laser and collected using a 379/28 bandpass filter. BUV395 has an excitation peak at 348 nm and an emission peak at 395. BUV395's fairly unique excitation and emission spectrum causes relatively little spillover into other common detectors and conflicts with very few other commonly used fluorophores. An alternative to BUV395 for flow cytometry is StarBright UltraViolet 400. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. These dyes were optimized for use with multicolor flow cytometry. BUV395 forms the backbone of the family, as all other members are tandems that use it as the acceptor, thus making them excitable by the same 355 nm UV laser.
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Brilliant Ultraviolet 395 Excitation and Emission Spectra