CD19 / PE-Cy7 / HIB19
CD19 / PE-Cy7 / HIB19
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 982410 (View supplier product page) | |
| Size | 500UL | |
| Price | $455.00 | |
| Antigen | CD19 | |
| Clone | HIB19 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | PE-Cy7 | |
| Target Species | Human | |
| Applications | FC | |
| Description | PE/Cyanine7 anti-human CD19 |
About CD19 and PE-Cy7
| CD19 | This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| PE-Cy7 | PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter. PE-CY7 has an excitation peak at 565 nm and an emission peak at 778 nm, and is a suitable alternative to PE-Vio®770 (Miltenyi) and cFluor BYG781 (Cytek). |
Images
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Typical results from human peripheral blood lymphocytes stained either with HIB19 PE/Fire™ 780 used at 5 µL/test (filled histogram) or with an isotype control (open histogram). (PE/Fire™ 780 is detected in the same channel as PE/Cy®7 |
Citations
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Reviews & Ratings
PE-Cy7 Excitation and Emission Spectra
$455.00
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