IgM Secondary Antibody / Brilliant Violet 711 / MHM-88
IgM Secondary Antibody / Brilliant Violet 711 / MHM-88
Citations:
(7)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 314540 (View supplier product page) | |
| Size | 100T | |
| Price | $446.00 | |
| Antigen | IgM | |
| Clone | MHM-88 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Brilliant Violet 711 | |
| Target Species | Human | |
| Applications | FC | |
| Description | Brilliant Violet 711 anti-human IgM |
About IgM and Brilliant Violet 711
| IgM | Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010] | |
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| Brilliant Violet 711 | Brilliant™ Violet 711 (BV711) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 710/50 bandpass filter. BV711 has an excitation peak at 405 nm and an emission peak at 711 nm. BV711 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Isolated human peripheral blood mononuclear cells were stained with CD19 PE and anti-human IgM (clone MHM-88) Brilliant Violet 711™ (top) or mouse IgG1, κ Brilliant Violet 711™ isotype control (bottom). |
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Citations
| PMID 24778443 | ||
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| Additional Sources |
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Reviews & Ratings
Brilliant Violet 711 Excitation and Emission Spectra
$446.00
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