CD31 / Brilliant Violet 421 / 390

Citations: (22)
Product Details
Supplier BioLegend
Catalog #: 102424 (View supplier product page)
Size 50 μg
Price $273.00
Antigen CD31
Clone 390
Host Rat
Isotype IgG2a κ
Conjugate Brilliant Violet 421
Target Species Mouse
Applications FC
Description Brilliant Violet 421 anti-mouse CD31
About CD31 and Brilliant Violet 421
CD31 The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Brilliant Violet 421 Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD31 Brilliant Violet 421 BioLegend antibody anti target:Mouse host:Rat isotype:IgG2a κ clone:390

C57BL/6 mouse splenocytes were stained with CD31 (clone 390) Brilliant Violet 421™ (open histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

Citations
PMID 19074419
Additional
Sources
Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra