FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP2-54452APC (View supplier product page)
	Size	100 ul
	Price	$519.00
	Antigen	ENO2
	Clone	ENO2/1375
	Host	Mouse
	Isotype	IgG2b κ
	Conjugate	APC
	Target Species	Human, Mouse, Rat
	Applications	FC
	Description	Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

		Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [Allophycocyanin] [NBP2-54452APC] - An intracellular stain was performed on SH-SY5Y cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
		Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [Allophycocyanin] [NBP2-54452APC] - An intracellular stain was performed on HeLa cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.