CD31 (PECAM-1) Brilliant Violet 421 (M89D3, Mouse) - Reactivity: Human
| Description |
The M89D3 monoclonal antibody specifically recognizes CD31, which is also known as platelet endothelial cell adhesion molecule -1 (PECAM-1), a single chain, type I transmembrane protein of approximately 130-140 kDa. CD31 is a member of the immunoglobulin supergene family. Its extracellular region is composed of 574 amino acids and contains six C2-type Ig domains, and the intracellular region is composed of 118 residues. CD31 (PECAM-1) is expressed on endothelial cells, platelets, monocytes, neutrophils and NK cells. It has also been observed on subsets of T cells, but not on circulating B cells. It has been implicated in a number of cellular phenomena, including vascular wound healing, angiogenesis, and transendothelial migration of leukocytes and platelet aggregation in inflammatory responses. |
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| Conjugate | Brilliant Violet 421 | |
| Clone | M89D3 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | BD Biosciences | |
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Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.
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