B7-H4 / Brilliant Violet 786 / MIH43

Product Details
Supplier BD Biosciences
Catalog #: 742960 (View supplier product page)
Size 50 μg
Price $538.00
Antigen B7-H4
Clone MIH43
Host Mouse
Isotype IgG1 κ
Conjugate Brilliant Violet 786
Target Species Human
Applications FC
Description Hu B7-H4 BV786 MIH43 50ug
About B7-H4 and Brilliant Violet 786
B7-H4 This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Brilliant Violet 786 Brilliant™ Violet 786 (BV786) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV750 has an excitation peak at 405 nm and an emission peak at 786 nm. Due to its unique Stokes shift, few dyes have identical or near identical excitation and emission spectrums, but the nearest alternatives are Qdot® 800 and SuperBright 780 (ThermoFisher), and Brilliant™ Violet 785 (BioLegend). What's the difference between BV785 and BV786? BV785 is a BioLegend color, while BV786 is a BD Biosciences color, but they exhibit the same spectral properties. This dye exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV786 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
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Brilliant Violet 786 Excitation and Emission Spectra