CD11b Monoclonal / FITC / REA713

Citations: (2)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-110-610 (View supplier product page)
Size 30 tests in 60 µL
Price $90.00
Antigen CD11b
Clone REA713
Host Human
Isotype IgG1
Conjugate FITC
Target Species Human
Applications FC, Mass Cytometry
Description Clone REA713 recognizes the human CD11b antigen (Mac-1 α; integrin αM chain), which is part of the CD11b/CD18 heterodimer (Mac-1 α, Mβ2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X. In humans, CD11b is strongly expressed on myeloid cells and weakly expressed on natural killer (NK) cells and some activated lymphocytes as well as on microglia in the brain. | Additional information: Clone REA713 displays negligible binding to Fc receptors.
About CD11b and FITC
CD11b This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Citations
PMID 1346576
PMID 20660248
PMID 24776637
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FITC Excitation and Emission Spectra