IFN-gamma / FITC / 43-11

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-31044F (View supplier product page)
Size 100 μl
Price $429.00
Antigen IFN-gamma
Clone 43-11
Host Mouse
Isotype IgG1 κ
Conjugate FITC
Target Species Human
Applications ELISA
Description This antibody recognizes both the recombinant and the natural form of human interferon-gamma (IFN-gamma) (1). This antibody does not react with human IL-4, IFN-alpha or acid inactivated IFN-gamma, and it shows only a minimal reactivity with heatinactivated IFN-g (<0.05%) (1).
About IFN-gamma and FITC
IFN-gamma This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra