CD235a Monoclonal / Unconjugated / JC159
CD235a Monoclonal / Unconjugated / JC159
Product Details
| Supplier | Thermo Fisher Scientific | |
|---|---|---|
| Catalog #: | MA5-12484 (View supplier product page) | |
| Size | 500 μl | |
| Price | $410.00 | |
| Antigen | CD235a | |
| Clone | JC159 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Unconjugated | |
| Target Species | Human, Rat | |
| Applications | WB, FC, IHC-P | |
| Description | CD235a Monoclonal Antibody (JC159) |
About CD235a and Purified
| CD235a | Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008] |
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Images
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Flow cytometry analysis of CD235a/Glycophorin A in K562 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD235a/Glycophorin A monoclonal antibody (Product # MA5-12484) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis. |
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Flow cytometry analysis of CD235a/Glycophorin A in rat bone marrow cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD235a/Glycophorin A monoclonal antibody (Product # MA5-12484) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis. |
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Flow cytometry analysis of CD235a/Glycophorin A in rat bone marrow cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD235a/Glycophorin A monoclonal antibody (Product # MA5-12484) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis. |
Citations
| PMID 24399013 | ||
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| Additional Sources |
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