Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-ATM pS1981 (clone 10H11.E12) antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1 - lysates from untreated Hela cells; Lane 2 - lysates from Hela cells treated with UV.

HeLa cells were stained with purified anti-ATM Phospho (Ser 1981) (clone 10H11.E12) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled with green. Nuclei were stained with DAPI (blue).