BACE subcellular localization in rat primary cortical neurons detected by BACE1 antibody B690 used at a 1:300 dilution. Photo courtesy of H. Xu, The Burnham Institute.

On the left, stable N2a cells overexpressing human BACE1 are immunprecipitated with B690 at a 1:250 dilution. The IP is detected on the blot with GP45 anti-BACE1. On the right, anti-BACE1 B690 used at 1:1,000 detects 2 bands on a standard Western blot of N2a cell lysates stably expressing human BACE1. Photo courtesy of H. Xu, The Burnham Institute.