FluoroFinder

	Supplier	Novus Biologicals, a Bio-Techne brand
	Catalog #:	NBP2-50082 (View supplier product page)
	Size	50 ug
	Price	$309.00
	Antigen	Fibroblasts
	Clone	TE-7
	Host	Mouse
	Isotype	IgG1
	Conjugate	Unconjugated
	Target Species	Human, Mouse
	Applications	ICC/IF, FC, IHC-P, IHC
	Description	The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.

		Immunocytochemistry: Fibroblasts Antibody (TE-7) [NBP2-50082] - MRC-5 human embryonic lung fibroblast cell line, fixation with PFA 4%, blocking with PBS 1X + 1% BSA + 0.1% Tween, primary antibody used at 1:50 in blocking buffer, O/N. Secondary antibody: goat anti mouse Alexa Fluor 594 for 1 hour at RT. Signal was detected by fluorescence microscopy at 40X. This image was submitted via customer review.
		Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082] - Paraffin-embedded gastrocnemius section from a patient with myopathic condition was performed overnight at 4 degrees C using 10 ug/mL.