p53 / Alexa Fluor 647 / PAB 1802

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NB100-2789AF647 (View supplier product page)
Size 100 μl
Price $399.00
Antigen p53
Clone PAB 1802
Host Mouse
Isotype IgG1
Conjugate Alexa Fluor 647
Target Species Human, Mouse
Applications WB, ELISA, IHC-Fr, IHC-P, IHC
Description PAB 1802 cross reacts with the murine epitope in C-terminal region between residues 306-393 in both wild type & mutant forms.
About p53 and Alexa Fluor 647
p53 This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
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Alexa Fluor 647 Excitation and Emission Spectra