p27 KIP 1 / DyLight 405 / DCS-72.F6 + KIP1/769

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-44497V (View supplier product page)
Size 100 μl
Price $429.00
Antigen p27 KIP 1
Clone DCS-72.F6 + KIP1/769
Host Mouse
Isotype IgG1 κ/IgG1 κ
Conjugate DyLight 405
Target Species Human, Monkey, Mouse, Rat
Applications IHC-P, WB, FC, IHC
Description Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
About p27 KIP 1 and DyLight 405
p27 KIP 1 This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
DyLight 405 DyLight™ 405 has an excitation peak at 400 nm and an emission peak at 420 nm and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. DyLight™ 405 is most commonly used in flow cytometery and fluorescence microscopy applications.
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DyLight 405 Excitation and Emission Spectra