CD19 / PE / HIB19
CD19 / PE / HIB19
Citations:
(11)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 982402 (View supplier product page) | |
| Size | 500UL | |
| Price | $315.00 | |
| Antigen | CD19 | |
| Clone | HIB19 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | PE | |
| Target Species | Human | |
| Applications | FC | |
| Description | PE anti-human CD19 |
About CD19 and PE
| CD19 | This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| PE | Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts. |
Images
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Typical results from human peripheral blood lymphocytes stained either with HIB19 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram). |
Citations
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Reviews & Ratings
PE Excitation and Emission Spectra
$315.00
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