CD160 Monoclonal / PE-Vio770 / REA669

Citations: (3)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-110-159 (View supplier product page)
Size 30 μg in 1 ml
Price $175.00
Antigen CD160
Clone REA669
Host Human
Isotype IgG1
Conjugate PE-Vio770
Target Species Mouse
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Description Clone REA669 recognizes the mouse CD160 antigen, which is also known as BY55. CD160 is a 27 kDa glycoprotein which is expressed at the cell surface as a tightly disulfide-linked multimer. It binds both classical and non-classical MHC class I molecules. CD160 is a ligand for HVEM (herpes virus entry mediator), which attenuates TCR-mediated signaling. Furthermore it enhances proliferation of activated CD8+ T cells and regulates NK cell activation. | Additional information: Clone REA669 displays negligible binding to Fc receptors.
About CD160 and PE-Vio770
CD160 CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]
PE-Vio770 PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
Citations
PMID 9743336
PMID 16764942
PMID 19426226
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PE-Vio770 Excitation and Emission Spectra