CD55 / Alexa Fluor 647 / F4-29D9
CD55 / Alexa Fluor 647 / F4-29D9
Product Details
| Supplier | Novus Biologicals, a Bio-Techne brand | |
|---|---|---|
| Catalog #: | NBP2-47963AF647 (View supplier product page) | |
| Size | 0.1 ml | |
| Price | $519.00 | |
| Antigen | CD55 | |
| Clone | F4-29D9 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Alexa Fluor 647 | |
| Target Species | Human | |
| Applications | FC | |
| Description | Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). |
About CD55 and Alexa Fluor 647
| CD55 | This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014] | |
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| Alexa Fluor 647 | Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. |
Images
![CD55 Alexa Fluor 647 Novus Biologicals, a Bio-Techne brand antibody anti target:Human host:Mouse isotype:IgG1 κ clone:F4-29D9 Flow Cytometry: CD55/DAF Antibody (F4-29D9) [Alexa Fluor (R) 647] [NBP2-47963AF647] - A surface s...](http://images.novusbio.com/images2/CD55-DAF-Antibody-(F4-29D9)-[Alexa-Fluor-(R)-647]-Flow-Cytometry-NBP2-47963AF647-img0004.jpg)
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Flow Cytometry: CD55/DAF Antibody (F4-29D9) [Alexa Fluor (R) 647] [NBP2-47963AF647] - A surface stain was performed on HT-29 cells with CD55/DAF (F4-29D9) antibody NBP2-47963AF647 and a matched isotype control NBP1-97005AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. |
Citations
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Reviews & Ratings
Alexa Fluor 647 Excitation and Emission Spectra
$519.00
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