CD86 NovaFluor Blue 760 (IT2.2, Mouse) - Reactivity: Human

About CD86
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

About NovaFluor Blue 760
NovaFluor Blue 760 dye has a unique emission spectrum when excited by the 488 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Blue 760 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 760 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Benefits of NovaFluor Blue 760 dye include: 1) Narrow emission spectrum and minimal cross-laser excitation for better resolution 2) Should be paired with moderately expressed antigens to minimize spillover 3) Spectrally unique and can be used to fill unused detectors in conventional flow cytometry 4) Produces stable fluorescence and is compatible with all buffers tested