Perforin / Alexa Fluor 594 / dG9
Perforin / Alexa Fluor 594 / dG9
Citations:
(1)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 308124 (View supplier product page) | |
| Size | 100 μg | |
| Price | $288.00 | |
| Antigen | Perforin | |
| Clone | dG9 | |
| Host | Mouse | |
| Isotype | IgG2b κ | |
| Conjugate | Alexa Fluor 594 | |
| Target Species | Human | |
| Applications | ICC | |
| Description | Alexa Fluor 594 anti-human Perforin |
About Perforin and Alexa Fluor 594
| Perforin | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] | |
|---|---|---|
| Alexa Fluor 594 | Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive. |
Images
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Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml Perforin (clone dG9) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for one hour. The nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. |
Citations
| PMID 19704116 | ||
|---|---|---|
| Additional Sources |
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Reviews & Ratings
Alexa Fluor 594 Excitation and Emission Spectra
$288.00
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