FluoroFinder

	Supplier	BioLegend
	Catalog #:	405326 (View supplier product page)
	Size	100 μg
	Price	$112.00
	Antigen	IgG
	Clone	Poly4053
	Host	Goat
	Isotype	IgG
	Conjugate	Alexa Fluor 594
	Target Species	Mouse
	Applications	IHC-Fr, ICC, IHC-P
	Description	Alexa Fluor 594 Goat anti-mouse IgG (minimal x-reactivity)

	IgG	This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
	Alexa Fluor 594	Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive.

		Frozen human tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD19 (clone SJ25C1) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
		Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD309 (clone A16085I) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
		HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of purified anti-human EGFR (clone AY13), followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red). Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.